The pictures you provided really makes your blab post amazing to look at! These bacteria were removed from the starter plate, did not have any plasmid added to them, and were replated on an LB plate. AP Lab 8: pGLO Transformation Lab Introduction Hypothesis: If we test the resistance to ampicillin in the bacteria, then the +pGLO samples will be much more resistant to the ampicillin than the –pGLO samples. 500 micrograms of CaCl2. On plate 4 any bacteria that has been transformed by taking up the pGREEN plasmid is now able to grow in the presence of the antibiotic since the plasmid also contains the gene allowing for antibiotic resistance.The colonies seem to be white but will  fluoresce green under UV light. In the pGLO lab, scientist were able to clone the GFP gene from a jellyfish known as Aequorea victoria, and from this they were able to make a recombinant plasmid, which is called pGLO. I appreciate you taking the time to read and find any mistakes. Bacteria which resemble the non-transformed will be found on the LB/ (-) pGLO plate. Purpose: The purpose of this lab was to study transformation and the effect that integrating certain genes into a typical E. Coli bacteria would have on the cell. Paper #2 Outline - Grade: B+ Essay #1 Final Draft - Grade: B+ Essay #2 Final Draft - Grade: A Essay #2 Outline - Grade: A pGLO Transformation Lab Report Organic Lab Report: Separation Of Hexane And Toluene Of Liquids Off season strength and conditioning for rugby 毛共筆Ch1-6-1 - econ Literature review Assignment in APA format Sample/practice exam September 2016, questions and answers … Here I discuss a popular bacterial transformation lab using the pGLO plasmid. 2) +pGLO LB/AMP/Ara Had Growth, (did Not Glow Under UV Light). Ampicillin is an antibiotic that kills E. coli, so if E. coli, so if E. coli cells contain the ampicillin-resistance gene, the cells can survive exposure to ampicillin since the ampicillin-resistance gene encodes an enzyme that … Hypothesis: If bacteria with +pGLO plasmids that are resistant to the antibiotic ampicillin and have the gene for GFP, colonies will survive and grow on the transformation plates that have LB/amp. I appreciate your time and consideration (: Nice job Stephanie. See science manual Bacterial Transformation Lab for complete list of materials and procedures. On the other hand the transformation plates contained bacteria resistant to the antibiotic which caused the colonies to survive and grow. Keep up the wonderful work! Proudly, we did not come across any sources of error. The goal of the lab is to get the bacteria to intake and express the pGLO gene and produce the protein, which will fluoresces green under the presence of ultraviolet light. If the transformed bacteria display the desired characteristic then we have successfully performed genetic transformation. There are colonies because the pGLO contains the plasmid, which allows the bacteria to survive and become resistant to the ampicillin. The lux opero Good job on your lab Steph! Bacterial transformation is the easiest type of genetic transformation to create in a lab due to the single celled nature of bacteria. Transformation •The pGlo Plasmid contains the following –A gene for GFP –A gene for antibiotic resistance –Regulation of the GFP gene. THe Lb/amp (pGLO -) plate will have no growth, the LB (pGLO -) plate will have a "lawn" of growth (meaning colonies covering the entire surface), the 3) -pGLO LB/AMP Had No Growth, (glowed Under UV Light). Thanks Chris! Cell/DNA mix is plated on nutrient agar with antibiotic. My group had the amazing opportunity to analyze our control and transformation plates be successful and transform but some groups did not have that opportunity due to decreasing their time or forgetting some steps to the experiment. Furthermore we will require plasmid in order to aid in the transformation process. ... pglo Transformation lab - Biology Forum Hypothesis- If the +pGLO solution is added to two of the four plates, and the –pGLO solution, which Crush It! Transformation. In this lab the engineered pGLO plasmid is incorporated into E. Coli bacteria, and adds the genes which code for the proteins GFP and beta lactamase to the modified bacteria’s genome. Taking up of DNA from the environment by bacterial cells. Other than that, your lab is very nice! I took your advice and fixed up my website. Transforming E. Coli with pGLO Plasmids, a Lab Day One Transformation Background: Transformation is a process of transferring genetic information from one organism to another. However I dont see your answer for the analysis questions (pre-lab and post-lab). Genetic Transformation of E. Coli with the Green Fluorescent Protein Kaelyn … More specifically, a previously prepared pGLO plasmid–which consisted of the gene to be cloned–was used to transform non-pathogenic bacteria. In this case, if the plate with +pGLO LB/amp/ara and +pGLO LB/amp have colonies of bacteria, and the colonies on the +pGLO LB/amp/ara plate … Genetic transformation is an active uptake of free DNA by a bacterial cell and the incorporation of the genetic information. In bacteria, a small circular piece of DNA known as a plasmid (Table … No need to be fancy, just an overview. Pglo transformation lab answers ABSTRACT: In this laboratory there are several plates containing with different combinations of LB agar, ampiallin and arabinosis. What is Bacterial Transformation? 4) -pGLO LB Had Growth, (did Not Glow Under UV Light). To add, the transformation plate with +DNA/+AMP/+IPTG was extremely successful growing colonie and also 5 glowing conies that fluoresces green under the UV light. The pGLO Plasmid. pGLO Lab By: Yi Yu. It's Not Supposed to Be This Way: Finding Unexpected Strength When Disappointments Leave You Shattered, Can't Hurt Me: Master Your Mind and Defy the Odds, I'll Be Gone in the Dark: One Woman's Obsessive Search for the Golden State Killer, 85% found this document useful (20 votes), 85% found this document useful, Mark this document as useful, 15% found this document not useful, Mark this document as not useful, Save AP Lab #6: pGLO Transformation Lab For Later. The hypothesis for the second plate is that bacteria will grow and glow because the gene for pGLO is introduced with sugar arabinose to effectively turn it on. Data Tables for plant growth and transformation: On plate 1 since there is no antibiotic present the wild-type bacteria grew normally and formed a "lawn" across the whole plate. What is Bacterial Transformation? Big Nate: What's a Little Noogie Between Friends? Experimental Procedures. More specifically, we used a previously prepared pGLO plasmid to be used to transform non-pathogenic bacteria E-Coli. Bio-Rad's pGLO Bacterial Transformation Kit is the classic kit for teaching the central dogma and the basics of genetic engineering. Thank you so much Fate! –Examples: Digestive system, immune system, skeletal system….. •Since each cell contains the same In this bacterial transformation lab activity, students use the pGLO plasmid to transform bacteria to express green fluorescent protein (GFP) from the bioluminescent jellyfish Aequorea victoria , which causes the bacteria to glow green under UV light. This process has many useful benefits when used correctly in different organisms. • Transformation • The pGLO Plasmid • Experimental Procedures • Extension Activities. Our alternate hypothesis for this experiment will be that the pGLO DNA will incorporate in the e. coli DNA and produce new traits. The bacteria will still grow although the ampacillin (which normally kills bacteria) is present because the pGLO gene also acts as a resistant to antibiotics. It's pretty easy to follow and I like how you used your creativity with the pictures on your procedures and everything else. To complete this task, we transformed E-Coli so that it would glow despite the presence of… In conclusion the experiment was successful, we determined transformation efficiency and results of genetic transformation. Abstract Plasmids are pieces of circular DNA that are in bacteria which can code for genes that can cause the bacteria to have extra “features”.With the pGLO plasmid this extra “feature” causes bacteria to glow (from the Green Fluorescent Protein that was inserted) and also has resistance to ampicillin (from the beta-lactamase protein). We completely every step successfully and were even helping other classmates gather up some information they did not successfully complete themselves. Results +pGLO LB/Amp. ...Bacterial Transformation Lab Report Backround: The plasmid pGLO contains an antibiotic-resistance gene, ampR, and the GFP gene is regulated by the control region of the ara operon. The reason for this experiment is that we wanted to determine the conditions of the bacteria that would glow. Abstract: The topic of this research involved the occurrence of genetic transformation in bacteria (E. Coli). I also think you should add measurements and quantities to your materials. What also could have happened is the students could have added pFluorogreen to the “-DNA” and the “+DNA”. The general process by which foreign DNA is introduced into a cell is called transformation.There are several ways to transform DNA into an E. coli cell, but the most common way is by making the cells competent. Cells which were not treated with DNA (-pGLO) should not be expressing the ampicillin resistance gene and will not grow on the LB/amp plates. No colonies were formed, showed no growth with the presence of AMP, On plate 300-500 colonies have grown and appear to be white without under the UV light. We proved our hypothesis correct that bacteria resistant to antibiotic ampicillin and have the gene for gFP colonies will survive and grow on the transformation plates. Also we concluded that the -pGlo bacteria without the presence of the plasmid could not survive with the ampicillin. However, several recent reports have shown that E. coli can express modest genetic competence in certain conditions that may arise in its environment. This is because the pGLO plasmid contains genes that give colonies of bacteria immunity to ampicillin, so they develop an ampicillin resistance. In addition, +pGLO bacteria on a plate with LB/amp/ara will grow and glow green under UV light because of the inclusion of arabinose. Summary: The pGLO Bacterial Transformation Lab is based off the molecular biology AP Biology lab involving transformation.The progression from DNA- RNA- protein- trait is shown with the bacteria E. Coli. If bacteria with +PGlo plasmids that are resistant to antibiotic, and have the gene for gFP then the colonies will survive and grow on the transformation plate that have LB(recovery broth) and amp. If bacteria with +PGlo plasmids that are resistant to antibiotic ampicillin and have the gene for gFP then the colonies will survive and grow on the transformation plate that have LB (recovery broth) and amp. In this lab, we tested the genetic transformation in bacteria. Thus, they are virtually identical to the non-transformed starter. Many of these mistakes can lead to no colonies forming or the experiment not being able to visualize the difference between the controls and the experiments. Write something about yourself. Escherichia coli is not assumed to be naturally transformable. : Why Now Is the Time to Cash in on Your Passion, Secrets of the Millionaire Mind: Mastering the Inner Game of Wealth, The Life-Changing Magic of Tidying Up: The Japanese Art of Decluttering and Organizing. This is how a lot of students got their tubes mixed up or switched around. pGLO™ Bacterial Transformation Kit Bio-Rad pGLO Kit Advantages •Standards-based •Comprehensive curricula for inquiry-based investigations •Compatible with 50 minute class periods •Serves entire class of 32 students (up to 4 students per group) •Cost-effective •Success in … HYPOTHESIS: 3/20/2016. This lab also explored the effect that certain environments would have on the bacteria, including those containing antibiotics or certain sugar molecules, as well as how the introduced gene would interact with these environments. Only cells which obtained plasmid DNA will grow… and glow. Taking up of DNA from the environment by bacterial cells. "Competent" cells have the ability to take up DNA molecules from the environment. I can see that you really took your time. Thank you Stacey! 6 Comments. Make sure your hypothesis is in the "if..then.." format, but VERY creative. Extension Activities. This lead to no growth on the controls. Click again to see term Background/Objectives/Hypothesis: Genetic transformation is a process that primarily is inserting new DNA into an organism to change that organism’s trait. HYPOTHESIS:-pGLO/LB plate (the control): Without the pGLO plasmid, the bacteria inside the plate will grow, since the bacteria are resistant to the antibiotics. It really did help thank you again! AP Bio pGLO Transformation Formal Lab Report Essay Sample. Bacterial Transformation With pGLO bacterial transformation, students learn about genetic engineering as they transform a non-virulent laboratory strain of Escherichia coli (E. coli) with the pGLO plasmid. Chapter 17/18: pGLO Transformation Lab PURPOSE The purpose of this lab is to learn all about genetic transformation through recombinant DNA by taking from one organism and injecting it into another one with the help of a plasmid. I will be sure to fix and change that. Overview. Factors that can be contributed to this fact is not adding CaCl2 solution into  just the “-DNA.” Also, maybe they did not leave the tubes incubated for the correct amount of time. Question: PGLO Transformation Lab Results: 1) +pGLO LB/AMP Had Growth (did Not Glow Under UV Light). Create your own unique website with customizable templates. The hypothesis for the first plate is that bacteria will grow, however it will not glow even though the pGLO gene is introduced because there is no arabinose to effectively activate the gene. Has a beige color +pGLO … Overview. View pGLO Transformation Lab.docx from BIOLOGY MISC at Temple University. To dispose of contaminated material: Immerse all disposable pipets, tubes, and loops that have come in contact with bacteria in 10% bleach solution for at least 20 minutes before draining, rinsing and disposing of in the trash. How is the pGLO plasmid introduced into the E. coli cell? 49 colonies. Abstract The purpose of this lab was to insert genes that would make E. coli resistant to ampicillin and to glow. Gene Regulation •Different types of cells produce different types of protein depending on their function. In this experiment plasmids, are inserted into a host E. coli cell. In this lab, bacteria was transformed by inserting DNA for Green Fluorescent Proteins. Cells which were treated with DNA (+pGLO) should contain the pGLO plasmid and should express the ampicillin resistance gene—the corresponding LB/amp plate will contain transformed bacterial colonies. Although the E. coli strain used in these experiments has been rendered non-pathogenic, it is important to teach the students good sterile technique and safe disposal of bacteria. Bacterial Transformation Lab. Any possible changes to be made if this experiment was to be complete is label tubes with group’s initials when incubated in the ice to separate from the other classmates’ tubes. I really enjoyed going over your lab Stephanie! Great job again! When lab is complete, collect all petri dis… The bacteria will also not die although ampacillin is present because, alike to the first plate, the pGLO assists the bacteria in … In lab groups, we genetically engineered the bacteria with pGLO plasmids containing specific codes for GFP (green fluorescent protein) and Ampicillin, an antibiotic, resistance. The procedure involves the CaCl 2 /heat shock method, which is a standard technique used in many research and biomanufacturing laboratories.